Tandem Affinity Purification

Engelsk definition

A technique used to isolate specific interacting partners of MACROMOLECULAR COMPLEXES that have been assembled with a tagged component (bait) that interacts with other partners in the complex. The interacting bait component is labeled with a dual-affinity tag that allows for capture of the complex by IMMUNOPRECIPITATION, followed by purification of only the partners of the complex interacting with the bait by affinity chromatography.

Svenska synonymer

Inga svenska synonymer finns.

Engelska synonymer

Affinity Purification, Tandem Tandem Affinity Chromatography Affinity Chromatography, Tandem Chromatography, Tandem Affinity Tandem Affinity Chromatographies